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FRANK M. POLACK, MD

Arch Ophthalmol. 1966;75(5):659-660.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Cells which are in the period of synthesis before mitosis incorporate tritiated thymidine to form desoxyribonucleic acid (DNA).^1,2 It has been assumed that the tritium incorporated into the cell nuclei persists for the life of the cells,² but halving of the isotope would occur with each cell division. Cells of tissues with a low mitotic index, such as those of the corneal endothelium,³ should demonstrate the possibility of indefinite persistence of the isotope.

The corneal endothelium of eyes of adult animals could be labeled only during the regeneration which followed mechanical trauma.⁴ Corneas labeled in this fashion by other investigators were reported to retain their label for at least five months.⁵ In our studies of persistence of donor cells in corneal homografts,⁶ destruction of corneal cells by freezing was found to be more advantageous than other kinds of trauma to the cornea. Endothelial cells . . . [Full Text PDF of this Article]

Author Affiliations
New York

From the Corneal Center, Columbia-Presbyterian Medical Center, New York.

Footnotes
Submitted for publication Sept 20, 1965.

Reprint requests to 635 W 165th St, New York, NY 10032 (Dr. Polack).

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