This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Citing articles on Web of Science (23)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

J. MANNAGH; A. RAY IRVING, Jr., MD.

Arch Ophthalmol. 1965;74(6):847-849.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

The primary culture of rabbit corneal endothelium after isolation by trypsinization has been reported.¹ Although this technique was uniformly successful in rabbits and monkeys, identical experiments upon human eyes failed with rare exception. A modification in technique has afforded success in 18 consecutive experiments on human corneas and, therefore, is thought worthy of reporting.

This paper describes the modified technique for isolation and the primary growth of human corneal endothelium in tissue culture and presents observations of cell activity and the appearance of the endothelial mitochondria. A brief summary of the isolation and growth techniques which were unsuccessful is also reported.

Materials and Methods

The studies utilized human eyes which were not required for corneal transplantation. The eyes, varying from 28 to 70 years-of-age, were removed under surgically aseptic conditions within six hours after death, and washed with 0.5% ophthalmic Neosporin* solution. Cell culture was established 24 to 72 . . . [Full Text PDF of this Article]

Author Affiliations
Los Angeles

From the Estelle Doheny Eye Foundation, Los Angeles.

Footnotes
Submitted for publication June 14, 1965.

Reprint requests to 272 s Lake St, Los Angeles, Calif 90057 (Mrs. Jean Mannagh).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?