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M. B. WHEELER, AB; C. V. HARDING, PhD; W. L. HUGHES, PhD

Arch Ophthalmol. 1964;71(6):861-864.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Previous studies have shown that maintenance of the rabbit lens in a perfusion culture system for two to three days can effect an increase in the number of cells undergoing mitosis and DNA synthesis (thymidine incorporation) in the central area of the lens epithelium.^1,2 This "activation" of DNA synthesis and mitosis occurs in an area of the epithelial layer which ordinarily shows very little or no mitotic activity.³ The use of measurements of the incorporation of I¹³¹-labeled idoxuridine (5-iodo-2'-deoxyuridine; IDU), an analogue of thymidine,^4,14 as an index of cellular proliferation in certain systems has been suggested.^5-8,15 The facility of detecting the -ray emission of tracer amounts of I¹³¹ offers certain advantages, for quantitative measurements, over more commonly used compounds such as C¹⁴- and H³-thymidine. Also, there is the possibility of sequential measurements on living tissue. The present paper shows that . . . [Full Text PDF of this Article]

Author Affiliations
New York

Departments of ophthalmology and physiology, College of Physicians and Surgeons, Columbia University, and the Brookhaven National Laboratory.

Footnotes
Submitted for publication Oct 25, 1963.

Supported in part by United States Atomic Energy Commission contract No. AT (30-1)2456, and by a Public Health Service research career program award No. NB-K6-1805, from the National Institute of Neurological Diseases and Blindness.

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