This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

JULES L. BAUM, MD

Arch Ophthalmol. 1963;70(1):59-68.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Introduction

The demonstration of the localization and activity of various respiratory enzymes is helpful in the study of tissue metabolism. It is the purpose of this paper to identify and localize the activity of multiple dehydrogenases and diaphorases in the cornea of the adult rabbit and rat, utilizing nitro-blue tetrazolium (nitro-BT) as an intracellular biologic indicator. The basic principle of dehydrogenase histochemistry lies in the ability of the tetrazolium salt to intercept hydrogen or hydrogen electrons from the biologic system with a consequent reduction to a deeply colored formazan pigment.¹ A detailed account of the biochemical events may be found elsewhere.^2-9

To date, very few histochemical tetrazolium studies of the cornea have been reported. In 1954, Jaeger,¹⁰ utilizing the earliest tetrazolium salt, triphenyl tetrazolium chloride (TTC), reported dehydrogenase activity in bovine cornea homogenates by spectrophotometric measurements. Calculations were based on formazan/corneal dry weight. When added to the . . . [Full Text PDF of this Article]

Author Affiliations
New York

From the Department of Ophthalmology, New York University School of Medicine.

Footnotes
Submitted for publication July 23, 1962.

This study was supported by Research Grant B-1951 from the National Institute of Neurological Diseases and Blindness, United States Public Health Service.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati     What's this?