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CALVIN HANNA, Ph.D.; JAMES E. O'BRIEN, Ph.D.

Arch Ophthalmol. 1961;66(3):362-365.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

When a corneal transplant "takes," a basic problem remains as to which corneal elements are replaced following the graft. The fate of the sulfated mucopolysaccharides of the transplant has been studied by several investigators using S-35^1,2 but methods are needed to specifically label and locate individual cells in order to study the movement of the cellular elements. In recent years thymidine-tritium has been used in studying the synthesis of desoxyribonucleic acid (DNA). Once incorporated into the nucleus the radioactive label is retained in the DNA which undergoes very slow turnover during the life of the cell. With highresolution autoradiographic film techniques it is possible to study the subsequent history of the labeled nucleus.³ The use of this method is limited because thymidine is taken up only during the premitotic doubling stage. In the case of the corneal epithelium, where there is rapid proliferation, the labeling problem is not . . . [Full Text PDF of this Article]

Author Affiliations
Burlington, Vt.

Footnotes
Submitted for publication April 28, 1961.

The study was supported in part by funds made available by a Senior Research Fellowship SF-156 (C.H.) from the U.S. Public Health Service and by a Grant-in-Aid from the Bob Hope Fight for Sight Fund of the National Council to Combat Blindness, Inc., New York City.

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