This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Citing articles on Web of Science (5)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

HESKEL M. HADDAD, M.D.; BERNARD BECKER, M.D.

Arch Ophthalmol. 1961;65(4):563-564.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Histochemical studies suggest the presence of acid mucopolysaccharides in a number of ocular tissues.^1-4 Sialic acids have been found in the aqueous and vitreous humors.⁵ In order to characterize the glycoproteins of other ocular tissues, analyses for sialic acids were made in different parts of the human eye.

Normal human eyes obtained at autopsy were provided by the eye bank. The anterior chamber was tapped first to collect the aqueous humor. The cornea was excised, and both epithelial and endothelial surfaces were scraped off. The lens was gently extracted, and its capsule was stripped off. The vitreous was collected in 2 portions: the more liquid anterior part and more viscous posterior fraction. The retina was stripped off the choroid and carefully cleared of adherent vitreous.

Each tissue was homogenized separately and heated with 0.1 N H₂SO₄ at 80 C for 1 hour. The sialic acids . . . [Full Text PDF of this Article]

Author Affiliations
St. Louis

From the Department of Ophthalmology and the Oscar Johnson Institute, Washington University School of Medicine.; Special Fellow, National Institute of Neurological Diseases and Blindness, National Institutes of Health, U.S. Public Health Service (Dr. Haddad).

Footnotes
Submitted for publication Dec. 15, 1960.

This investigation was supported in part by a research grant, B-1911, from the National Institute of Neurological Diseases and Blindness of the National Institutes of Health, U.S. Public Health Service.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?