This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Citing articles on Web of Science (4)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

LELAND F. WATTS, Jr., M.D.; CHARLES J. MARTIN, Ph.D.

Arch Ophthalmol. 1961;65(1):24-25.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Following the early reports by Barraquer¹ and Jenkins² on the use of -chymotrypsin in cataract surgery, ophthalmic surgeons have shown considerable interest in the further development of this new technique.

This communication details results that indicate by both biochemical assay and clinical experience, at least one commercial -chymotrypsin preparation * can be used repeatedly after solubilization as a chemical adjunct to cataract surgery.

Experimental Results

The enzyme preparation was solubilized with its accompanying diluent as per manufacturer's directions to give a 1:5,000 dilution (stock enzyme solution). All aliquot removals were performed aseptically.

In these experiments 2 separate samples of -chymotrypsin were used; one for stability studies at 25 C and the other for comparable purposes at 2 C. Residual enzymatic activity was determined at pH 8.0 and 30 C with either N-carbobenzoxy-L-tyrosine p-nitrophenyl ester (CTN) or N-acetyl-L tyrosine ethyl ester (ATEE) as substrates. . . . [Full Text PDF of this Article]

Author Affiliations
Pittsburgh

Department of Ophthalmology, University of Pittsburgh School of Medicine, Eye & Ear Hospital of Pittsburgh, and Biochemistry Department, School of Medicine, University of Pittsburgh.

Footnotes
Submitted for publication Aug. 22, 1960.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?