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Immunoelectrophoretic and Gel Diffusion Studies on Normal and Pathological Human Aqueous Humor

WALTER L. PERETZ, M.D.; THOMAS B. TOMASI, M.D.

Arch Ophthalmol. 1961;65(1):20-23.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Introduction

The application of immunological techniques such as immunoelectrophoresis and gel diffusion has been of considerable value in determining the distribution and characteristics of proteins in various body fluids.^1,2 Serum and urine have been the body fluids most frequently studied.

Other workers have noted difficulty in analysis of individual samples of aqueous humor.^3,4 This is due to the limited amount of fluid available and the small concentrations of protein present. For these reasons, concentrates or pools of several samples were often made prior to analysis.^5,6,7 In the present study the immunological techniques applicable to small volumes of fluid were applied to the analysis of individual specimens. Normal and pathological aqueous humor were both examined and a comparison made between the immunoelectrophoretic pattern of the patient's aqueous

Materials and Methods The 24 patients in this study were seen at The New York Hospital-Cornell Medical Center. Aqueous was obtained . . . [Full Text PDF of this Article]

Author Affiliations
New York

From the Department of Surgery (Ophthalmology), The New York Hospital-Cornell Medical Center (Dr. Peretz) and The Rockefeller Institute (Dr. Tomasi).

Footnotes
Submitted for publication Aug. 17, 1960.

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