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Part III

BERNARD SCHWARTZ, M.D., Ph.D.

AMA Arch Ophthalmol. 1960;63(4):625-642.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Introduction

The ideal perfusion medium for the culture of the rabbit lens is one that closely imitates the aqueous in its composition. Since the anterior chamber aqueous is modified by the metabolic activity of the tissues bordering the anterior chamber, it was felt that the most physiological medium would be a synthetic fluid based on the composition of the posterior chamber aqueous. This fluid resembles more closely the primary aqueous that first encounters the lens. A series of studies designed to develop a synthetic lens culture medium based on posterior aqueous humor analyses will be discussed.

Primary Constituents of the Synthetic Medium

The main constituents of the synthetic medium are salts and nonelectrolytes patterned after the analysis of the posterior aqueous humor by Kinsey (1953). Table 1 indicates the values of Kinsey's data com

pared to the levels of constituents in the synthetic perfusion medium. Kinsey's concentrations are based on . . . [Full Text PDF of this Article]

Author Affiliations
Iowa City

From the Departments of Physiology and Ophthalmology, College of Medicine, State University of Iowa.

Footnotes
Submitted for publication Nov. 23, 1959.

Present address of author: Division of Ophthalmology, Department of Surgery, State University of New York, Downstate Medical Center, Brooklyn 3, N.Y.

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, in the Department of Physiology in the Graduate College of the State University of Iowa.

This study was supported in part by Grant B-1522 from the National Institute of Neurological Diseases and Blindness and was carried out during a tenure of a Special Clinical Traineeship (BT-322) National Institute of Neurological Diseases and Blindness, United States Public Health Service.

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