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An Analysis of the Illumination Technique of Cibis
ROBERT C. DREWS, M.D.
AMA Arch Ophthalmol. 1958;59(4):579-583.
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| Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings. |
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Cibis recently published an account of a new technique of illumination for microscopy.1 Figure 1 illustrates some of the major differences between microscopy with transmitted and Cibis illumination. Note how details which are not visible with ordinary illumination suddenly appear, such as zones of discontinuity in the lens. There are also striking color changes in the cornea, iris pigment, and so forth. Subtle color differences appear, for instance between ciliary muscle and surrounding connective tissue. In the present work I have attempted to analyze this method in order to be better able to interpret what is seen, to plan further applications, and to be able perhaps to extend the technique.
The Production of Color
Color in an object is produced by seven basic mechanisms: Light striking an object
. . . [Full Text PDF of this Article]
Author Affiliations
St. Louis
From the Department of Ophthalmology, Washington University School of Medicine and the Oscar Johnson Institute.
Footnotes
Received for publication Sept. 9, 1957.
This investigation was supported in part by a Fight for Sight Grant-in-Aid, No. 185, of the National Council to Combat Blindness, Inc., New York.
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