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I. Rate of Disappearance of Radio-Chromium-Tagged Red Cells

H. K. BOYER, Ph.D.; ANITA A. SURAN, M.S.; M. J. HOGAN, M.D.; K. G. SCOTT, Ph.D.; W. K. McEWEN, Ph.D.

AMA Arch Ophthalmol. 1958;59(2):232-234.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Simulated vitreous hemorrhages were produced in rabbits by injection of homologous blood tagged with radioactive chromium. The course of the hemorrhage was followed by measuring the rate of change of radioactivity of the eye and by visual and histologic methods.

Methods and Materials

Rabbit blood was collected in oxalated tubes. The erythrocytes were separated by centrifugation and thoroughly washed with isotonic saline. Radioactive sodium chromate (Na₂Cr⁵¹O₄) was used to label the hemoglobin of the cells by the procedure of Gray and Sterling¹ as modified by Reilly et al.2 The tagged erythrocytes were then either suspended in the original plasma to reconstitute whole blood or were laked in a volume of distilled water equal to the initial plasma volume.

Hemorrhage was simulated by injection, through a 30-gauge needle, of 0.10 ml. of this blood into the vitreous. Since -rays and soft x-rays from the radioactive chromium in . . . [Full Text PDF of this Article]

Author Affiliations
San Francisco

From the Francis I. Proctor Foundation for Research in Ophthalmology (Drs. Boyer, Hogan, and McEwen and Miss Suran) and the Radioactivity Research Center (Dr. Scott), University of California Medical Center.

Footnotes
Received for publication May 27, 1957.

This investigation was supported in part by Contract DA-49-007-MD-394 of the Department of the Army.

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