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Corticosteroids in the Aqueous Humor of the RabbitPaper Chromatography
HARRY GREEN, Ph.D.;
HENRY S. KROMAN, M.S.;
IRVING H. LEOPOLD, M.D.
AMA Arch Ophthalmol. 1955;54(6):853-857.
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In a previous investigation1 analysis of chloroform extracts of aqueous humor from normal rabbit eyes by the blue tetrazolium method indicated the presence of corticosteroids in quantities of the order of magnitude of 1-1.5 per milliliter. Since the blue tetrazolium method neither permits the identification of individual compounds in a mixture nor is specific only for oxidizable corticoids, the extracted aqueous humor was fractionated by paper partition chromatography and analyzed for corticosteroids. The results of this investigation strongly indicate that at least part of the blue-tetrazoliumpositive material present in aqueous humor is hydrocortisone.
MATERIALS
The following purified crystalline corticosteroids were donated by Sharp & Dohme, Division of Merck & Company, Inc.: 11-dehydrocorticosterone (A); corticosterone (B); 11-desoxy-17-hydroxycorticosterone (S); cortisone (11-dehydro-17hydroxycorticosterone, Compound E); hydrocortisone (17-hydroxycorticosterone, Compound F); hydrocortisone acetate; dihydrocortisone, and tetrahydrocortisone. Small aliquots of the samples were dissolved in absolute methanol in concentrations of 1 and 37 per microliter and
. . . [Full Text PDF of this Article]
Author Affiliations
Philadelphia
Department of Research, Wills Eye Hospital.
Footnotes
Received for publication Sept. 6, 1955.
This investigation was supported in part by a research grant from the National Institute of Neurological Diseases and Blindness, of the National Institutes of Health, U. S. Public Health Service.
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