This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Citing articles on Web of Science (17)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

A Preliminary Report

LIEUT. COL. JOEL N. McNAIR; COL. J. H. KING, Jr., MC

AMA Arch Ophthalmol. 1955;53(4):519-521.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

The operation of keratoplasty is frequently limited by the unavailability of donor corneal material. This report concerns preliminary experiments on the storage of cornea by dehydration and its successful use in lamellar transplantation in animals.

Many attempts have been made to store corneal grafts over long periods, and various methods have been tried in animals, without success. Katzin (1947),¹ Leopold and Adler (1947),² and Smelser and Ozanics (1946)³ all used rapid freezing in liquid nitrogen, with and without isopentane as an intermediate heat-transfer medium. In the method described in the first two papers, the grafts, after freezing, were dried in vacuo over P₂O₅ at -40 C. They were reconstituted before use, in most cases, by the addition of isotonic sodium chloride solution. Smelser and Ozanics did not dry their materials but stored them at -195 C for periods of from one hour up to . . . [Full Text PDF of this Article]

Author Affiliations
U. S. Army

Ophthalmology Service, Walter Reed Army Medical Center (Lieut. Col. McNair). Chief, Ophthalmology Service, Walter Reed Army Medical Center (Col. King), Washington 12, D. C.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?