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II. Gelatinous Protein of Vitreous Body

ROGER G. YOUNG, Ph.D.; HAROLD H. WILLIAMS, Ph.D.

AMA Arch Ophthalmol. 1954;51(5):593-595.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

OF THE proteins which are present in the vitreous body, the gelatinous material seems to be most significant. It has been termed vitrein,¹ or residual protein,² and a recent preparation of the structural protein has been called vitrosin.² Krause¹ purified the protein by filtration, followed by treatment with papain, and recorded some data on its chemical properties. It was the purpose of this investigation to prepare the gelatinous protein of the vitreous, by methods calculated to preserve its native state as much as possible, and to determine, in part, its chemical composition.

METHODS

Vitreous bodies from freshly killed cattle were removed and placed on a Buchner funnel without filter paper. Very gentle stirring with a horizontal glass rod allowed the viscous liquid to drain through. About 90% of the original volume was removed, leaving a thick gel.

Inevitably, some denaturation occurred during filtration. The opalescent fibers . . . [Full Text PDF of this Article]

Author Affiliations
ITHACA, N. Y.

From the School of Nutrition and the Department of Biochemistry and Nutrition, Cornell University.

Footnotes
Supported in part by a grant to Cornell University by William C. Geer.

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