This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

JOHN BELLOWS, M.D., Ph.D.; JOHN COOPER, Ph.D.; HENRY B. BULL, Ph.D.

Arch Ophthal. 1943;30(5):621-625.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

The electrophoretic technic of Tiselius¹ has been used by a number of workers to study the proteins in normal and in pathologic human serums.² In this method, a boundary is formed in a specially designed U tube between a solution of proteins in a buffer and an ultrafiltrate of the solution. The application of a potential gradient across the boundary causes the original single boundary to separate into a series of boundaries corresponding to the number of electrophoretically discrete protein fractions present in the original mixture (certain anomalous boundaries being excluded). The boundaries move toward the electrode of the sign opposite the charge on the proteins forming the boundaries. By use of a lens system developed by Philpot,³ and later modified by Svensson,⁴ the positions of the boundaries can be visualized and the concentrations of the protein components determined. In short, the components of a mixture . . . [Full Text PDF of this Article]

Author Affiliations
CHICAGO

From the Departments of Ophthalmology and Physiological Chemistry, Northwestern University Medical School.

Snyder Fellow in Ophthalmic Research.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati     What's this?