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Arch Ophthalmol. 1998;116:976-977.

In the article entitled "In Vivo Delivery of Phosphorothioate Oligonucleotides Into Murine Retina," published in the March issue of the ARCHIVES (Arch Ophthalmol. 1998;116:342-348), Figure 1 and Figure 2 were poorly reproduced. The appropriately reproduced Figure 1 and Figure 2 now appear here. The journal regrets the error.

View larger version (106K): [in this window] [in a new window] Figure 1. Cross sections of retina intravitreally injected with naked fluorescein isothiocyanate (FITC)–labeled phosphorothioate oligonucleotides (S-ODNs) or hemagglutinating virus of Japan (HVJ) liposomes containing FITC-labeled S-ODNs. A through C, Eyes injected with naked FITC-labeled S-ODNs. A, 3 µmol/L, 1 hour after injection. B, 30 µmol/L, 1 hour after injection. Note low fluorescence in retinal blood vessels (arrows). C, 30 µmol/L, 1 day after injection. Note high fluorescence in the retinal pigment epithelial cells with nuclei that appeared as dark spots (arrows). D, Control eyes injected with HVJ liposomes containing nonlabeled S-ODNs. E through H, Eyes injected with HVJ liposomes containing 3-µmol/L FITC-labeled S-ODNs. E, One hour after injection. Note high fluorescence in retinal blood vessels (arrows). F, Three days after injection. G, Seven days after injection. Note high fluorescence in the retinal pigment epithelial cells, where nuclei appeared as dark spots (arrows). GCL indicates ganglion cell layer; ONL, outer nuclear layer; bar, 25 µm.

View larger version (109K): [in this window] [in a new window] Figure 2. Flat-mounted retina focused on the ganglion cell layer. A through C, Eyes injected with naked fluorescein isothiocyanate (FITC)–labeled phosphorothioate oligonucleotides (S-ODNs). A, 3 µmol/L, 1 hour after injection. B, 30 µmol/L, 1 hour after injection. C, 30 µmol/L, 1 day after injection. This fluorescence on day 1 was detected only in small localized areas. D and E, Eyes injected with hemagglutinating virus of Japan (HVJ) liposomes containing 3-µmol/L FITC-labeled S-ODNs. D, 1 hour after injection. E, 5 days after injection. Note that many cells showed fluorescence seemingly in the cytoplasm, but scattered cells showed fluorescence in the nuclei. F, Retina stained with 5% propidium iodide for 2 minutes. G and H, Low-power fluorescent micrographs showing widespread distribution of fluorescence on day 1 in eyes injected with HVJ liposomes (G) and rapid elimination of fluorescence in eyes injected with 30-µmol/L naked FITC-labeled S-ODNs at the same time (H). Bar indicates 25 µm (A through F); 100 µm (G and H).