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Katrin Tefs, PhD; Louise Kobelt; Maike Ziegler; Boris Hügle, MD; Volker Schuster, MD

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Leyva-Castillo et al¹ described a Mexican patient with ligneous conjunctivitis. Hemostaseologic studies revealed severe hypoplasminogenemia (type I plasminogen deficiency). Mutation screening of the plasminogen (PLG) gene (chromosome 6q26) suggested a 14–base pair deletion in exon 5 of the PLG gene (deleted sequence, GAACTACTGCAGGA). However, this deletion has already been described before as the result of a methodical error: accidental coamplification of sequences of the plasminogen-related gene B (NCBI accession number AC093616.5) located on chromosome 2.^2-3 Furthermore, polymerase chain reaction amplification of PLG gene exons 1, 3, 4, 5, 13, 16, and 17 may lead to "false" distinct nucleotide exchanges owing to coamplified PLG homologous genes.³ Optimizing polymerase chain reaction conditions by, for example, increasing the annealing temperature or changing the primers will probably lead to the disappearance of the alleged deletion and false nucleotide exchanges.^3-4 . . . [Full Text of this Article]

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RELATED LETTER

Difficulties in Mutation Screening of the Plasminogen (PLG) Gene in Patients With Ligneous Conjunctivitis and Severe Hypoplasminogenemia—Reply
Juan M. Leyva-Castillo, Humberto Cavazos-Adame, Augusto Rojas-Martínez, Martín César Fernández-Espinoza, and Rocío Ortiz-López
Arch Ophthalmol. 2007;125(9):1303.
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RELATED ARTICLE

Ligneous Conjunctivitis in a Mexican Patient With a Mutation in the Plasminogen (PLG) Gene
Juan M. Leyva-Castillo, Humberto Cavazos-Adame, Augusto Rojas-Martínez, Roberto López-Garza, Martin Cesar Fernandez Espinoza, and Rocío Ortiz-López
Arch Ophthalmol. 2006;124(10):1500-1501.
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