Wide field specular microscopy of excised donor corneas

C. V. Roberts, H. D. Rosskothen and C. J. Koester

Routine examination of the endothelium of eye bank excised corneas in the McCarey-Kaufman storage medium has been accomplished by using a new bottle cap with an optical quality window. A sterile system is maintained as the cornea remains in the bottle within which it is sent to the surgeon, without handling or manipulation of the tissue. In combination with the wide field specular microscope in the noncontact mode, endothelial cell measurements and qualitative information about the endothelial surface are now available.