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Bartly J. Mondino, MD; Helen V. Ratajczak, PhD; Daniel B. Goldberg, MD; David J. Schanzlin, MD; Stuart I. Brown, MD

Arch Ophthalmol. 1980;98(2):346-349.

Abstract
• Activation of complement by either the classical or alternate pathway may be involved in corneal inflammation. This study was undertaken to determine whether the normal human cornea contains components for both classical and alternate pathway activation of complement. Direct immunofluorescence of corneas from human donors using fluorescein-labeled antiserums was used to demonstrate C1q, C3, C4, and C5. The C1q component (the recognition unit of the classical pathway and largest complement component) was found in the periphery of the cornea. Normal donor corneas were also eluted in phosphate-buffered saline at 4 °C for one to four days. Ouchterlony plates, in which the corneal eluate was reacted against antiserums to complement components, disclosed the presence of C1q, C3, C4, C5, properdin, and properdin factor B. Plasminogen was also found. Radial immunodiffusion was used to obtain estimates of the concentrations of C3, C4, and C5 in the cornea.

Author Affiliations
From the Department of Ophthalmology, University of Pittsburgh School of Medicine and Eye and Ear Hospital, Pittsburgh.

Footnotes
Accepted for publication May 22, 1979.

Reprint requests to the Department of Ophthalmology, Eye and Ear Hospital, 230 Lothrop St, Pittsburgh, PA 15213 (Dr Mondino).

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