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Hemolytic Complement Activity in Normal Human Donor Corneas
Bartly J. Mondino, MD;
Dana B. Hoffman
Arch Ophthalmol. 1980;98(11):2041-2044.
Abstract
• Normal human donor corneas were minced into small fragments and eluted for 16 to 23 hours at 4 °C. The corneal eluates were then studied for hemolytic complement activity of C1, C4, C2, C3, C5, C6, and C7 with 50% hemolysis (CH50) of sensitized sheep RBCs. Sera from ten normal volunteers were also assayed for hemolytic complement activity in CH50 units per milliliter. For each complement component, the mean hemolytic activity in corneas was compared with the mean hemolytic activity in sera. These comparisons suggest that molecular weight may be a factor in determining the concentration of complement components in the cornea. The present study provides normal values of hemolytic complement activity for further studies of complement consumption in corneal diseases.
Author Affiliations
From the Department of Ophthalmology, University of Pittsburgh School of Medicine, and Eye and Ear Hospital, Pittsburgh.
Footnotes
Accepted for publication March 11, 1980.
Reprint requests to Department of Ophthalmology, Eye and Ear Hospital, 230 Lothrop St, Pittsburgh, PA 15213 (Dr Mondino).
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