Quantitative analysis of the Callender classification of uveal melanoma cells
J. W. Gamel and I. W. McLean
Six experienced ophthalmic pathologists independently classified 90 uveal
melanoma cells according to the Callender classification. They frequently
disagreed among themselves, but at least four of the six agreed on the
classification of 77 cells. In an effort to delineate those cytologic
features that are most useful in the application of Callender
classification, 12 factors were evaluated for each of these cells.
Multivariate analysis (MVA) proved that six factors (chromatin clumping,
minimum nuclear diameter, nucleolar diameter, chromatin margination,
maximum nuclear diameter, and abundant cytoplasm) were useful for
determining the cell type selected by the majority of pathologists,
although some cells could not be sharply classified. It was concluded that
uveal melanoma cells occupy a continuous cytologic spectrum, from
benign-appearing spindle A cells to very malignant-appearing epithelioid
cells.
