Examination and Photography of Donor Corneal Endothelium

doi:10.1001/archopht.1976.03910040573017

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Vol. 94 No. 10, October 1976

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Examination and Photography of Donor Corneal Endothelium

William M. Bourne, MD

Arch Ophthalmol. 1976;94(10):1799-1800.

Abstract

• In a method for the routine examination of the endotheliumof donor corneas before transplantation, the donor endothelialcells are examined and photographed as they undergo preservationor culture in liquid media. A specular microscope with a modifiedobjective lens is used (working distance, approximately 9 mm).The donor corneas are kept in a special storage container thatallows a direct view of the endothelium from outside the container.The endothelium of any cornea preserved in a liquid medium maythus be examined and photographed at high magnification withoutopening the storage container.

Author Affiliations

From the Mayo Clinic and Mayo Foundation, Rochester, Minn.

Footnotes

Accepted for publication April 12, 1976.

Reprint requests to Mayo Clinic and Mayo Foundation, 200 SecondSt SW, Rochester, MN 55901 (Dr Bourne).

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