 |
|
Repair of DNA in Xeroderma Pigmentosum Conjunctiva
David A. Newsome, MD;
Kenneth H. Kraemer, MD;
Jay H. Robbins, MD
Arch Ophthalmol. 1975;93(8):660-662.
Abstract
Xeroderma pigmentosum (XP) is an autosomal recessive disease with tumor formation on sun-exposed areas of the skin and eyes. Cells from most XP patients are deficient in repairing DNA damaged by ultraviolet (UV) light as shown by a reduced rate of tritiated thymidine (3HTdR) incorporation during their DNA repair synthesis.
We have studied such repair synthesis in conjunctival cells from an XP patient with a conjunctival epithelioma and from normal cadaver conjunctiva. Cultured conjunctival cells were irradiated with UV light and then incubated with 3HTdR. Autoradiograms were prepared and showed that UV radiation induced a considerably slower rate of DNA repair synthesis in the XP cells than in normal cells. Many of the ocular abnormalities of XP, including tumor formation, may be the result of this defective DNA repair process.
Author Affiliations
From the Laboratory of Vision Research, National Eye Institute, and the Dermatology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md.
Footnotes
Submitted for publication June 11, 1974.
Reprint requests to Massachusetts Eye and Ear Infirmary, 243 Charles St, Boston, MA 02114 (Dr. Newsome).
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Malignant Melanoma of the Iris in Xeroderma Pigmentosum
Johnson et al.
Arch Ophthalmol 1989;107:402-407.
ABSTRACT
Reduced DNA Repair in Cultured Melanocytes and Nevus Cells From a Patient With Xeroderma Pigmentosum
Kraemer et al.
Arch Dermatol 1989;125:263-268.
ABSTRACT
|
