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Loss of Protein Kinase C in Knockout Mice and Increased Retinal Sensitivity to Hyperbaric Oxygen
Vladimir V. Yevseyenkov, MS;
Satyabrata Das, MS;
Dingbo Lin, PhD;
Lloyd Willard, BS;
Harriet Davidson, DVM, PhD;
Ari Sitaramayya, PhD;
Frank J. Giblin, PhD;
L. Dang, BS;
Dolores J. Takemoto, PhD
Arch Ophthalmol. 2009;127(4):500-506.
Objective To determine if loss of protein kinase C (PKC) results in increased structural damage to the retina by hyperbaric oxygen (HBO), a treatment used for several ocular disorders.
Methods Six-week-old mice were exposed in vivo to 100% HBO 3 times a week for 8 weeks. Eyes were dissected, fixed, embedded in Epon, sectioned, stained with toluidine blue O, and examined by light microscopy.
Results The thicknesses of the inner nuclear and ganglion cell layers were increased. Destruction of the outer plexiform layer was observed in the retinas of the PKC-knockout mice relative to control mice. Exposure to HBO caused significant degradation of the retina in knockout mice compared with control mice. Damage to the outer segments of the photoreceptor layer and ganglion cell layer was apparent in central retinas of HBO-treated knockout mice.
Conclusions Protein kinase C–knockout mice had increased retinal sensitivity to HBO. Results demonstrate that PKC protects retinas from HBO damage.
Clinical Relevance Care should be taken in treating patients with HBO, particularly if they have a genetic disease, such as spinocerebellar ataxia type 14, a condition in which the PKC is mutated and nonfunctional.
Author Affiliations: Department of Biochemistry (Mssrs Yevseyenkov and Das, and Drs Lin and Takemoto), and College of Veterinary Medicine (Mr Willard and Dr Davidson), Kansas State University, Manhattan; and Eye Research Institute, Oakland University, Rochester, Michigan (Drs Sitaramayya and Giblin, and Ms Dang).
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