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Early Effects of Triamcinolone on Vascular Endothelial Growth Factor and Endostatin in Human Choroidal Neovascularization
Olcay Tatar, MD;
Kei Shinoda, MD, PhD;
Edwin Kaiserling, MD;
Grazia Pertile, MD;
Claus Eckardt, MD;
Andreas Mohr, MD;
Efdal Yoeruek, MD;
Peter Szurman, MD;
Karl U. Bartz-Schmidt, MD;
Salvatore Grisanti, MD
Arch Ophthalmol. 2008;126(2):193-199.
Objective To evaluate the early effects of triamcinolone acetonide as monotherapy or as an adjuvant to ocular verteporfin photodynamic therapy (PDT) on angiogenesis in human choroidal neovascularization (CNV) secondary to age-related macular degeneration.
Methods Retrospective review of an interventional series of 55 patients who underwent CNV extraction. Eleven patients were treated with intravitreal triamcinolone acetonide (4 mg) monotherapy (triamcinolone-treated CNV group [n = 5]) or with PDT–triamcinolone combination therapy (PDT–triamcinolone-treated CNV group [n = 6]) 3 to 9 days before surgery. Forty patients who underwent CNV extraction without previous therapy (control CNV group) and 4 patients who underwent CNV extraction 3 days after PDT (PDT CNV group) served as control subjects. The CNV samples were stained for CD34, endostatin, cytokeratin 18, and vascular endothelial growth factor (VEGF).
Results Vascular endothelial growth factor expression was stronger in the PDT CNV samples (P < .001), triamcinolone CNV samples (P = .01), and PDT–triamcinolone CNV samples (P = .007) compared with the control CNV samples. There were no statistically significant differences in VEGF expression among the PDT CNV samples, triamcinolone CNV samples, and PDT–triamcinolone CNV samples. Endostatin expression was weaker in the PDT CNV samples than in the control CNV samples (P = .008). Endostatin expression was stronger in the triamcinolone CNV samples and the PDT–triamcinolone CNV samples compared with the control CNV samples (P = .001 and P < .001, respectively) and the PDT CNV samples (P < .001 for both).
Conclusion To some extent, triamcinolone monotherapy seems to exert its angiogenesis inhibitory effects on CNV by enhancing endostatin expression rather than by suppressing VEGF expression.
Author Affiliations: Centre for Ophthalmology, University Eye Hospital, Eberhard-Karls University (Drs Tatar, Yoeruek, Szurman, Bartz-Schmidt, and Grisanti), and Department of Pathology, University of Tuebingen (Dr Kaiserling), Tuebingen, Augenklinik der Staedtischen Kliniken, Frankfurt am Main (Dr Eckardt), and St Joseph Stift Augenklinik, Bremen (Dr Mohr), Germany; Laboratory of Visual Physiology, National Institute of Sensory Organs, Tokyo, Japan (Dr Shinoda); and Department of Ophthalmology, Sacro Cuore Hospital, Negrar, Italy (Dr Pertile).
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