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A Histopathologic and Immunohistochemical Study

Beeran Meghpara, BA; Hiroshi Nakamura, MD; Marian Macsai, MD; Joel Sugar, MD; Ahmed Hidayat, MD; Beatrice Y. J. T. Yue, PhD; Deepak P. Edward, MD

Arch Ophthalmol. 2008;126(12):1655-1663.

Objective  To examine histopathologic and immunohistochemical features of human corneal buttons from patients who developed keratectasia after laser in situ keratomileusis (LASIK).

Methods  Five corneal buttons were obtained during penetrating keratoplasty from patients who developed keratectasia after LASIK. Histologic features were examined by hematoxylin-eosin staining using paraffin-embedded sections and by transmission electron microscopy. Immunostaining for ₁-proteinase inhibitor, Sp1, and matrix metalloproteinases 1, 2, and 3 was performed with 2 healthy corneas and 2 corneas with keratoconus as controls.

Results  Central stromal thinning was observed after hematoxylin-eosin staining in all corneas with keratectasia. No histologic features specific to keratoconus, including Bowman layer disruption, were identified in the corneas with keratectasia. By transmission electron microscopy, collagen fibril thinning and decreased interfibril distance were observed in the stromal bed. Immunostaining intensity and/or pattern for ₁-proteinase inhibitor and Sp1 in the corneas with keratectasia was comparable to that of healthy corneas and differed from that in the corneas with keratoconus. No significant staining with anti–matrix metalloproteinases 1, 2, and 3 antibodies was observed in either the corneas with keratectasia or the healthy corneas.

Conclusions  Histologic findings suggest that post-LASIK keratectasia results in collagen fibril thinning and decreased interfibril distance within the residual stromal bed. Discrepant results between keratectasia and keratoconus suggest that the pathogenesis of the 2 conditions differ.

Author Affiliations: Departments of Ophthalmology, University of Illinois at Chicago (Mr Meghpara and Drs Sugar and Yue) and Northwestern University (Dr Macsai), Chicago, Illinois; Summa Health Systems, Akron, Ohio (Drs Nakamura and Edward); and Armed Forces Institute of Pathology, Washington, DC (Dr Hidayat).

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