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Yoshiyuki Satake, MD, PhD; Murat Dogru, MD, PhD; Gen-Yuki Yamane, DDS, PhD; Shigeru Kinoshita, MD, PhD; Kazuo Tsubota, MD; Jun Shimazaki, MD

Arch Ophthalmol. 2008;126(1):23-28.

Objective  To determine the barrier function and cytologic features of ocular surface epithelium after autologous cultivated oral mucosal epithelial transplantation in a prospective observational study.

Methods  The status of the epithelium in 4 eyes with limbal stem cell deficiency was studied preoperatively and postoperatively. We used an impression method to determine the cytologic features and anterior fluorophotometry to determine barrier function.

Results  Impression cytology showed nonkeratinized, squamous, polygonal, cohesive cells with a low nuclear to cytoplasmic cell ratio and no goblet cells, corresponding to cultivated oral mucosal epithelium, at up to 16 months after surgery. In some cases, the epithelium displayed a mixture of oral mucosal and conjunctival epithelium, especially in cases with a longer postoperative period. Central epithelial permeability remained persistently high throughout the follow-up period, regardless of the epithelial phenotype.

Conclusions  Cultivated oral mucosal epithelial cells were observed to survive for more than 1 year after transplantation, with gradual replacement by conjunctival epithelium in some cases. Decreased barrier function of the transplanted epithelium may have prognostic implications, suggesting the presence of oral mucosal epithelium long after surgery.

Author Affiliations: Departments of Ophthalmology (Drs Satake, Dogru, Tsubota, and Shimazaki) and Oral Medicine (Dr Yamane), Tokyo Dental College, Chiba, Japan; Department of Ophthalmology, Kyoto Prefectural University of Medicine, Graduate School of Medicine, Kyoto, Japan (Dr Kinoshita); and Johnson & Johnson Department of Ocular Surface and Visual Optics (Dr Dogru) and Department of Ophthalmology (Drs Tsubota and Shimazaki), Keio University School of Medicine, Tokyo, Japan.

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