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Preclinical Study of Brilliant Blue G as an Adjunct for Capsular Staining

Toshio Hisatomi, MD, PhD; Hiroshi Enaida, MD, PhD; Hiroyoshi Matsumoto, MD; Tadahisa Kagimoto, MD; Akifumi Ueno, MD; Yasuaki Hata, MD, PhD; Toshiaki Kubota, MD, PhD; Yoshinobu Goto, MD, PhD; Tatsuro Ishibashi, MD, PhD

Arch Ophthalmol. 2006;124:514-519.

Objective  To evaluate the effectiveness and biocompatibility of brilliant blue G (BBG) for capsular visualization for continuous curvilinear capsulorrhexis.

Methods  The capsular staining ability of BBG was evaluated at graded concentrations of 10.0, 1.0, 0.5, 0.25, 0.1, and 0.01 mg/mL in enucleated pig's eyes. The biocompatibility of BBG was assessed in rat's eyes for 2 months. The eyes were analyzed using light, fluorescence, transmission electron, and scanning electron microscopy. TUNEL (terminal deoxynucleotidyl transferase–mediated biotin–deoxyuridine triphosphate nick-end labeling) was used to detect apoptotic cells, and endothelial cell counts were analyzed using scanning electron microscopy. The results were compared using indocyanine green and trypan blue.

Results  The BBG improved capsular visualization, and a complete capsulorrhexis could be performed. In the rat model, no apparent toxic effect was observed using biomicroscopy during 2 months. Histologically, BBG showed satisfactory biocompatibility. Apoptotic cell death of the endothelial cells was detected in only the trypan blue group. In contrast to BBG, indocyanine green and trypan blue showed degeneration of corneal endothelial cells using transmission and scanning electron microscopy.

Conclusion  The BBG contributed to better capsular visualization and caused no apparent complications to the corneal endothelium.

Clinical Relevance  The BBG is effective and safe capsular staining for continuous curvilinear capsulorrhexis.

Author Affiliations: Departments of Ophthalmology (Drs Hisatomi, Enaida, Matsumoto, Kagimoto, Ueno, Hata, and Ishibashi) and Neurophysiology (Dr Goto), Graduate School of Medical Sciences, Kyushu University, and Department of Ophthalmology, University of Occupational and Environmental Health (Dr Kubota), Fukuoka, Japan.

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