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Oxidative DNA Damage in the Human Trabecular Meshwork
Clinical Correlation in Patients With Primary Open-Angle Glaucoma
Sergio Claudio Saccà, MD;
Antonio Pascotto, MD;
Paola Camicione, MD;
Paolo Capris, MD;
Alberto Izzotti, MD, PhD
Arch Ophthalmol. 2005;123:458-463.
Objective To evaluate the intensity of oxidative molecular damage and its clinical correlations: visual field damage, intraocular pressure, age, and disease duration.
Methods DNA was extracted from human trabecular meshwork specimens collected from 17 glaucoma-affected patients using standard filtration surgery. Twenty-one specimens from healthy eyes collected for cornea transplants serve as controls. Oxidative DNA damage was evaluated by determining 8-hydroxy-2'-deoxyguanosine levels. All patients underwent a Humphrey 30-2 visual field examination and diurnal tonometry before surgery.
Results The mean ± SD DNA oxidative damage was 8.51 ± 5.44 and 1.75 ± 1.80 8-hydroxy-2'-deoxyguanosine molecules/105 normal nucleotides in patients with glaucoma and controls, respectively. A statistically significant correlation was found among human trabecular meshwork DNA oxidative damage, visual field damage, and intraocular pressure. No other statistically significant correlations were found.
Conclusions Oxidative stress may represent an important pathogenetic step in primary open-angle glaucoma because it could induce human trabecular meshwork degeneration, favoring an intraocular pressure increase, thus priming the glaucoma pathogenetic cascade.
Author Affiliations: Department of Neurosciences, Ophthalmology, and Genetics, Clinica Oculistica (Drs Saccà, Camicione, and Capris), and Department of Health Sciences, Section of Hygiene and Preventive Medicine (Dr Izzotti), University of Genoa, Genoa, Italy; and Casa di Cura GEPOS-Telese Terme (Dr Pascotto), Benevento, Italy.
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