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Experimental Study on Human Donor Corneas

Milenko Stojkovic, MD; Michael Küchle, MD; Berthold Seitz, MD, FEBO; Achim Langenbucher, PhD; Arne Viestenz, MD; Anja Viestenz, MD; Carmen Hofmann-Rummelt, MTA; Ursula Schlötzer-Schrehardt, PhD; Gottfried O. H. Naumann, MD

Arch Ophthalmol. 2003;121:1415-1422.

Objective  To assess the alterations in human donor corneal tissue induced by Q-switched erbium (Er):YAG laser corneal trephination.

Methods  Thirty human corneoscleral donor buttons unsuitable for transplantation were placed in an artificial chamber on an automated rotation device. Corneas were trephined with a Q-switched Er:YAG laser (wavelength, 2.94 µm; pulse duration, 400 nanoseconds) along (donor and recipient) aluminum silicate (ceramic) open masks. A spot diameter of 0.65 mm, energy setting of 50 mJ/pulse, and repetition rate of 5 Hz were used. Corneal thermal damage and cut regularity were quantitatively assessed in 24 corneas processed for light microscopy and by transmission and scanning electron microscopy.

Results  The stromal thermal damage was the highest (mean [SD], 8.0 [2.7] µm) at a 150-µm cut depth and decreased downward. Cut regularity was very good and did not significantly differ between donors and recipients. Scanning electron microscopy confirmed that the cuts were highly regular; transmission electron microscopy revealed 2 distinctive subzones within the stromal thermal damage zone.

Conclusions  Thermal damage induced by Q-switched Er:YAG nonmechanical corneal trephination was low, and the regularity of the cuts was very good.

Clinical Relevance  The Q-switched Er:YAG laser may have the potential to become an alternative to the excimer laser for nonmechanical penetrating keratoplasty.

From the Department of Ophthalmology, Friedrich Alexander Universität–Erlangen-Nürnberg, Erlangen, Germany.

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