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Complete Androgen Insensitivity Syndrome
Effect on Human Meibomian Gland Secretions
Benjamin D. Sullivan, BS;
James E. Evans;
Jennifer M. Cermak, PhD;
Kathleen L. Krenzer, OD, PhD;
M. Reza Dana, MD, MPH;
David A. Sullivan, PhD
Arch Ophthalmol. 2002;120:1689-1699.
Objective To determine whether androgen receptors affect the fatty acid profiles of neutral and polar lipids in human meibomian gland secretions.
Methods Meibomian gland secretion samples were obtained from both eyes of (1) women with complete androgen insensitivity syndrome, a condition characterized by dysfunctional androgen receptors, and (2) age-matched female and male controls. Samples were processed for high-performance liquid chromatography, mass spectrometry, or both and for analysis of the mass spectra of neutral and polar lipid fatty acid fragment ions by 3 different methods.
Results Androgen receptor dysfunction is associated with significant alterations in the appearance of numerous molecular species in the neutral and polar lipid fractions of meibomian gland secretions. The ability to detect these differences, and to assess their nature and extent, was facilitated by the use of several analytic approaches. Sex-related differences exist in the expression of a variety of neutral and, especially, polar fatty acid products in meibomian gland secretions.
Conclusions Androgens exert a significant effect on neutral and polar lipids in human meibomian gland secretions, and these hormonal effects may be mediated through androgen receptors.
From the Schepens Eye Research Institute (Mr Sullivan and Drs Cermak, Dana, and Sullivan), Brigham and Women's Hospital (Drs Cermak and Dana), the Department of Ophthalmology, Harvard Medical School (Drs Cermak, Dana and Sullivan), and the New England College of Optometry (Dr Krenzer), Boston, Mass; and the Shriver Center, University of Massachusetts Medical School, Waltham (Mr Evans).
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