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A Preliminary Study

Stefan Dithmar, MD; Dario Rusciano, PhD; Michael J. Lynn, MS; David H. Lawson, MD; Cheryl A. Armstrong, MD; Hans E. Grossniklaus, MD

Arch Ophthalmol. 2000;118:1085-1089.

Objectives  To investigate the treatment of metastasis from uveal melanoma and to test the effect of interferon (IFN) alfa-2b in a murine model.

Methods  The B16-LS9 tissue culture melanoma cells were inoculated into the posterior intraocular compartment of 3 groups of C57BL/6 mice. The inoculated eyes were enucleated at 9 days and the mice were euthanized at 26 days after inoculation; the site and number of metastases were determined using standard histologic techniques. Group 1 was the control group; group 2 was given 20,000 international units (IU) of IFN alfa-2b intramuscularly 12 hours before enucleation, and group 3 received daily injections of 20,000 IU of IFN alfa-2b intramuscularly starting 4 days before enucleation.

Results  Pulmonary metastases were detected in 57%, 33%, and 0% of groups 1, 2, and 3, respectively; hepatic micrometastases were detected only in group 1. These results showed a significant decrease in hepatic metastases in mice receiving IFN alfa-2b vs controls (P = .005).

Conclusion  Treatment with IFN alfa-2b results in decreased hepatic metastases from intraocular melanoma in a murine model.

From the Departments of Ophthalmology (Drs Grossniklaus and Rusciano), Medicine (Dr Lawson), and Dermatology (Dr Armstrong), and the School of Public Health (Mr Lynn), Emory University School of Medicine, Atlanta, Ga; and the Friedrich Miescher Institut, Basel, Switzerland (Dr Rusciano).

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