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Matrix Metalloproteinases and Tumor Necrosis Factor in Glaucomatous Optic Nerve Head

Xiaoming Yan, MD, PhD; Gülgün Tezel, MD; Martin B. Wax, MD; Deepak P. Edward, MD

Arch Ophthalmol. 2000;118:666-673.

Objective  To study expression and location of matrix metalloproteinases (MMPs) and tumor necrosis factor (TNF-) in glaucomatous optic nerve heads, which are known to be secreted in response to a variety of neuronal injury.

Methods  Four postmortem eyes from patients with primary open-angle glaucoma, 7 eyes from patients with normal-pressure glaucoma, and 4 eyes from age-matched normal donors were studied by immunohistochemistry. The sections of the optic nerve heads were examined after immunostaining with antibodies to MMPs (MMP-1, MMP-2, and MMP-3), TNF-, or TNF- receptor 1.

Results  The intensity of the immunostaining and the number of stained cells for MMPs, TNF-, or TNF- receptor 1 were greater in the glaucomatous optic nerve heads, particularly in eyes with normal-pressure glaucoma compared with age-matched controls. Positive immunostaining was observed in all regions of the glaucomatous optic nerve heads, but most prominently in the postlaminar region. Immunostaining was observed mainly in glial cells and their processes around the axons and blood vessels and in pial septae.

Conclusion  There is increased immunostaining for MMPs, TNF- and TNF- receptor 1 in the glaucomatous optic nerve head, which suggests increased expression of these proteins in glaucoma and thereby implies a role in the tissue remodeling and degenerative changes seen in glaucomatous optic nerve heads.

Clinical Relevance  The MMPs and TNF- may be components of astroglial activation that occurs in glaucomatous optic nerve heads. The biological alterations in the expression of these proteins may play a role in the progression of glaucomatous optic neuropathy.

From the Departments of Ophthalmology and Visual Sciences, University of Illinois at Chicago (Drs Yan and Edward) and Washington University School of Medicine, St Louis, Mo (Drs Tezel and Wax). The authors have no proprietary interest in any of the materials used in this study.

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