This Article  • Full text  • PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (10)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Ophthalmology, Other  • Alert me on articles by topic  Social Bookmarking   What's this?

Expression of FcRI, c-kit, ICAM-1, and IgE

James L. Stahl, PhD; Ellen B. Cook, BS; Frank M. Graziano, MD, PhD; Neal P. Barney, MD

Arch Ophthalmol. 1999;117:493-497.

Objective  To characterize the expression and regulation of conjunctival mast cell surface receptors important in allergic inflammation.

Methods  Mast cells were isolated from human conjunctival tissues of cadavers. Mast cell surface markers were identified using flow cytometry with antibodies to IgE, FcRI, c-kit, and intercellular adhesion molecule-1 (ICAM-1). We evaluated the effect of 24-hour tumor necrosis factor (TNF-) or interleukin 4 (IL-4) incubation on the expression of mast cell c-kit, ICAM-1, and surface-bound IgE.

Results  Staining of mast cells (c-kit and/or tryptase positive) yielded positive results for all of the variables measured. The intensity of mast cell c-kit staining increased with TNF- incubation, but decreased below that of unstimulated mast cells when incubated with IL-4. Anti–ICAM-1 and anti–IgE staining were increased over that of unstimulated cells when incubated with TNF- or IL-4.

Conclusions  In this model, TNF- up-regulates mast cell surface receptors and cell-bound IgE. Interleukin 4 up-regulates mast cell ICAM-1 and cell-bound IgE, but down-regulates c-kit.

Clinical Relevance  Conjunctival mast cells play a critical role in the pathogenesis of atopic ocular disease. Characterization of the expression and regulation of mast cell surface receptors is important to the development of potential novel treatments for ocular inflammation.

From the Departments of Medicine (Drs Stahl and Graziano and Ms Cook) and Ophthalmology and Visual Sciences (Dr Barney), School of Medicine, University of Wisconsin–Madison.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-{alpha}, TGF{beta}-1 and IL-6
Fifadara et al.
Int Immunol 2009;21:991-1001.
ABSTRACT | FULL TEXT