This Article  • Full text  • PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on ISI (19)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Ophthalmology, Other  • Alert me on articles by topic

Baohe Tian, MD; Cameron Millar, PhD; Paul L. Kaufman, MD; Alexander Bershadsky, PhD; Eitan Becker, PhD; Benjamin Geiger, PhD

Arch Ophthalmol. 1998;116:1070-1077.

Objectives  To determine the effects of H-7 on (1) iris and ciliary muscles (CMs) in living monkeys; (2) isolated monkey CM strips; (3) actomyosin contractility in cultured Swiss 3T3 cells.

Methods  (1) Pupillary diameter (calipers) and accommodation (refractometer) in living monkeys were measured after topical, intracameral, or intravitreal administration of H-7 followed by systemic pilocarpine hydrochloride. (2) Pilocarpine-induced contraction of isolated monkey CM strips following administration of H-7 was measured in a perfusion chamber. (3) Actomyosin contractility in Swiss 3T3 cells cultured on thin silicone rubber film was determined by measuring cell-induced film wrinkles before and after administration of H-7.

Results  Topical H-7 prevented anesthesia-induced miosis but did not affect resting refraction. Intracameral or intravitreal H-7 dilated the pupil and inhibited miotic but not accommodative responses to pilocarpine. H-7 inhibited pilocarpine-induced contraction of isolated monkey CM strips and reduced Swiss 3T3 cell contraction.

Conclusions  H-7 inhibits actin-based contractility in non-muscle cells and in monkey iris sphincter and CM. Under our in vivo experimental conditions, the effect on the iris predominates over that on the CM.

From the Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison (Drs Tian, Millar, and Kaufman), the Wisconsin Regional Primate Research Center (Dr Kaufman), Madison; and the Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel (Drs Bershadsky, Becker, and Geiger). University of Wisconsin and Weizmann Institute of Science have filed a related patent application; accordingly, Drs Kaufman and Geiger have a proprietary interest. Alcon Laboratories, Inc, Fort Worth, Tex has paid a fee to University of Wisconsin and Weizmann Institute of Science for an option to license this technology and has provided some financial support in this general area. Dr Kaufman has served as a paid consultant on unrelated issues for Alcon Laboratories and several other pharmaceutical corporations.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Nonmuscle myosin II is responsible for maintaining endothelial cell basal tone and stress fiber integrity
Goeckeler et al.
Am. J. Physiol. Cell Physiol. 2008;295:C994-C1006.
ABSTRACT | FULL TEXT  

Effect of Low-Dose Latrunculin B on Anterior Segment Physiologic Features in the Monkey Eye
Okka et al.
Arch Ophthalmol 2004;122:1482-1488.
ABSTRACT | FULL TEXT  

Effects of Rho-Associated Protein Kinase Inhibitor Y-27632 on Intraocular Pressure and Outflow Facility
Honjo et al.
IOVS 2001;42:137-144.
ABSTRACT | FULL TEXT  

Mediation of Calcium-Independent Contraction in Trabecular Meshwork through Protein Kinase C and Rho-A
Thieme et al.
IOVS 2000;41:4240-4246.
ABSTRACT | FULL TEXT  

H-7 Effects on the Structure and Fluid Conductance of Monkey Trabecular Meshwork
Sabanay et al.
Arch Ophthalmol 2000;118:955-962.
ABSTRACT | FULL TEXT  

The Effects of Protein Kinase C on Trabecular Meshwork and Ciliary Muscle Contractility
Thieme et al.
IOVS 1999;40:3254-3261.
ABSTRACT | FULL TEXT  

Molecular diversity of cell-matrix adhesions
Zamir et al.
J. Cell Sci. 1999;112:1655-1669.
ABSTRACT