This Article  • References  • Full text PDF  • Correction  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (115)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Cynthia A. Toth, MD; Drew G. Narayan; Stephen A. Boppart, MSEE; Michael R. Hee, MSEE; Jim G. Fujimoto, PhD; Reginald Birngruber, PhD; Clarence P. Cain, PhD; Cheryl D. DiCarlo, DVM; W. Patrick Roach, PhD

Arch Ophthalmol. 1997;115(11):1425-1428.

Abstract
Objective
To compare the cross-sectional images of primate retinal morphology obtained by optical coherence tomography (OCT) with light microscopy to determine the retinal components represented in OCT images.

Methods
Laser pulses were delivered to the retina to create small marker lesions in a Macaca mulatta. These lesions were used to align in vivo OCT scans and ex vivum histologic cross sections for image comparison.

Results
The OCT images demonstrated reproducible patterns of retinal morphology that corresponded to the location of retinal layers seen on light microscopic overlays. Layers of relative high reflectivity corresponded to horizontally aligned retinal components such as the nerve fiber layer and plexiform layers, as well as to the retinal pigment epithelium and choroid. In contrast, the nuclear layers and the photoreceptor inner and outer segments demonstrated relative low reflectivity by OCT.

Conclusions
Retinal morphology and macular OCT imaging correlate well, with alignment of areas of high and low reflectivity to specific retinal and choroidal elements. Resolution of retinal structures by OCT depends on the contrast in relative reflectivity of adjacent structures. Use of this tool will enable expanded study of retinal morphology, both normal and pathologic, as it evolves in vivo.

Author Affiliations
From Duke University Eye Center, Durham, NC (Dr Toth and Mr Narayan); Massachusetts Institute of Technology, Cambridge (Messrs Boppart and Hee and Dr Fujimoto); Medizinisches Laserzentrum, Lübeck, Germany (Dr Birngruber); TASC Inc, San Antonio, Tex (Dr Cain); Uniformed Services University of the Health Sciences, Bethesda, Md (Dr DiCarlo); and Air Force Office of Scientific Research, Boiling AFB, Washington, DC (Dr Roach).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Optical coherence tomography is less sensitive than visual evoked potentials in optic neuritis
Naismith et al.
Neurology 2009;73:46-52.
ABSTRACT | FULL TEXT  

Macular Thickness Measurements in Healthy Eyes Using Six Different Optical Coherence Tomography Instruments
Wolf-Schnurrbusch et al.
IOVS 2009;50:3432-3437.
ABSTRACT | FULL TEXT  

Optical coherence tomographic patterns in diabetic macular oedema: prediction of visual outcome after focal laser photocoagulation
Kim et al.
Br J Ophthalmol 2009;93:901-905.
ABSTRACT | FULL TEXT  

Optical coherence tomography differs in neuromyelitis optica compared with multiple sclerosis
Naismith et al.
Neurology 2009;72:1077-1082.
ABSTRACT | FULL TEXT  

Relationship of the Optical Coherence Tomography Signal to Underlying Retinal Histology in the Tree Shrew (Tupaia belangeri)
Abbott et al.
IOVS 2009;50:414-423.
ABSTRACT | FULL TEXT  

Retinal Peripapillary Nerve Fiber Layer Thickness in Neuromyelitis Optica
Merle et al.
IOVS 2008;49:4412-4417.
ABSTRACT | FULL TEXT  

Comparison of the optical coherence tomographic features of choroidal neovascular membranes in pathological myopia versus age-related macular degeneration, using quantitative subanalysis
Keane et al.
Br J Ophthalmol 2008;92:1081-1085.
ABSTRACT | FULL TEXT  

Automatic segmentation in three-dimensional analysis of fibrovascular pigmentepithelial detachment using high-definition optical coherence tomography
Ahlers et al.
Br J Ophthalmol 2008;92:197-203.
ABSTRACT | FULL TEXT  

The characteristic features of optical coherence tomography in posterior uveitis
Gallagher et al.
Br J Ophthalmol 2007;91:1680-1685.
ABSTRACT | FULL TEXT  

Reproducibility of Quantitative Optical Coherence Tomography Subanalysis in Neovascular Age-Related Macular Degeneration
Joeres et al.
IOVS 2007;48:4300-4307.
ABSTRACT | FULL TEXT  

The role of heredity in determining central retinal thickness
Liew et al.
Br J Ophthalmol 2007;91:1143-1147.
ABSTRACT | FULL TEXT  

Cozzarelli Prize Winner@;DELIM@;From the Cover: Muller cells are living optical fibers in the vertebrate retina
Franze et al.
Proc. Natl. Acad. Sci. USA 2007;104:8287-8292.
ABSTRACT | FULL TEXT  

Noninvasive Volumetric Imaging and Morphometry of the Rodent Retina with High-Speed, Ultrahigh-Resolution Optical Coherence Tomography
Srinivasan et al.
IOVS 2006;47:5522-5528.
ABSTRACT | FULL TEXT  

A reference standard for the measurement of macular oedema.
Goatman
Br J Ophthalmol 2006;90:1197-1202.
ABSTRACT | FULL TEXT  

Heritability of Macular Thickness Determined by Optical Coherence Tomography
Chamberlain et al.
IOVS 2006;47:336-340.
ABSTRACT | FULL TEXT  

Macular microholes: pathogenesis and natural history
Zambarakji et al.
Br J Ophthalmol 2005;89:189-193.
ABSTRACT | FULL TEXT  

Enhanced optical coherence tomography imaging by multiple scan averaging
Sander et al.
Br J Ophthalmol 2005;89:207-212.
ABSTRACT | FULL TEXT  

Evaluation of Heredity as a Determinant of Retinal Nerve Fiber Layer Thickness as Measured by Optical Coherence Tomography
Hougaard et al.
IOVS 2003;44:3011-3016.
ABSTRACT | FULL TEXT  

Morphological and functional analyses of adult onset vitelliform macular dystrophy
Saito et al.
Br J Ophthalmol 2003;87:758-762.
ABSTRACT | FULL TEXT  

Histologic Correlation of Pig Retina Radial Stratification with Ultrahigh-Resolution Optical Coherence Tomography
Gloesmann et al.
IOVS 2003;44:1696-1703.
ABSTRACT | FULL TEXT  

Topographical Thickness of the Epithelium and Total Cornea after Hydrogel and PMMA Contact Lens Wear with Eye Closure
Wang et al.
IOVS 2003;44:1070-1074.
ABSTRACT | FULL TEXT  

Decreased Visual Acuity Associated With Cystoid Macular Edema in Neovascular Age-related Macular Degeneration
Ting et al.
Arch Ophthalmol 2002;120:731-737.
ABSTRACT | FULL TEXT  

Noninvasive Imaging by Optical Coherence Tomography to Monitor Retinal Degeneration in the Mouse
Li et al.
IOVS 2001;42:2981-2989.
ABSTRACT | FULL TEXT  

Progressive Change of Optical Coherence Tomography Scans in Retinal Degeneration Slow Mice
Horio et al.
Arch Ophthalmol 2001;119:1329-1332.
ABSTRACT | FULL TEXT  

Optical coherence tomography of the vitreoretinal interface in macular hole formation
Tanner et al.
Br J Ophthalmol 2001;85:1092-1097.
ABSTRACT | FULL TEXT  

Reproducibility of Retinal Mapping Using Optical Coherence Tomography
Massin et al.
Arch Ophthalmol 2001;119:1135-1142.
ABSTRACT | FULL TEXT  

The Humphrey optical coherence tomography scanner: quantitative analysis and reproducibility study of the normal human retinal nerve fibre layer
Jones et al.
Br J Ophthalmol 2001;85:673-677.
ABSTRACT | FULL TEXT  

The Retinal Nerve Fiber Layer Thickness in Ocular Hypertensive, Normal, and Glaucomatous Eyes With Optical Coherence Tomography
Bowd et al.
Arch Ophthalmol 2000;118:22-26.
ABSTRACT | FULL TEXT  

The Interpretation of Optical Coherence Tomography Images of the Retina
Chauhan and Marshall
IOVS 1999;40:2332-2342.
ABSTRACT | FULL TEXT  

Macular Hole Formation: New Data Provided by Optical Coherence Tomography
Gaudric et al.
Arch Ophthalmol 1999;117:744-751.
ABSTRACT | FULL TEXT