You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | Access Rights | Sign In


  Vol. 114 No. 8, August 1996 TABLE OF CONTENTS
  Archives
  •  Online Features
  LABORATORY SCIENCES
 This Article
 •References
 •Full text PDF
 • Reply to article
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati Add to Twitter What's this?

A Mouse Model of Diabetic Retinopathy

Timothy S. Kern, PhD; Ronald L. Engerman, PhD

Arch Ophthalmol. 1996;114(8):986-990.


Abstract

Objective
To obtain a model of diabetic retinopathy to which modern methods of genetic engineering may be applied, by determining the response of 2 strains of mice to long-term galactose feeding.

Methods
Both C57BL/6 mice and BALB/c mice were fed each of 2 galactose-rich diets (30% and 50% galactose), and trypsin digests of their retinas were compared with those of controls at durations of up to 26 months.

Results
The mortality rate in galactose-fed animals was lower in C57BL/6 mice than in BALB/c mice, and both strains tolerated the 30% galactose diet significantly better than the 50% galactose diet. In C57BL/6 mice fed 30% galactose for 21 to 26 months, saccular microaneurysms were observed in the retina, together with significant increases in the thickness of capillary basement membrane and the prevalence of acellular capillaries and pericyte ghosts. The 50% galactose diet caused significantly more acellular capillaries than normal by 15 months, but excessive mortality precluded study at longer durations. The frequency of acellular capillaries also was greater than normal in BALB/c mice fed 30% galactose for 21 months. Retinal polyol levels in galactose-fed mice were found to be lower than those in galactosemic rats.

Conclusion
The mouse may provide an inexpensive model suitable for in vivo study of the pathogenesis of diabetic retinopathy using molecular biological techniques.



Author Affiliations

From the Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison.



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Retinal Ion Regulation in a Mouse Model of Diabetic Retinopathy: Natural History and the Effect of Cu/Zn Superoxide Dismutase Overexpression
Berkowitz et al.
IOVS 2009;50:2351-2358.
ABSTRACT | FULL TEXT  

Diabetic Retinopathy: Seeing Beyond Glucose-Induced Microvascular Disease
Antonetti et al.
Diabetes 2006;55:2401-2411.
ABSTRACT | FULL TEXT  

Vascular Damage in a Mouse Model of Diabetic Retinopathy: Relation to Neuronal and Glial Changes
Feit-Leichman et al.
IOVS 2005;46:4281-4287.
ABSTRACT | FULL TEXT  

Aldose Reductase Deficiency Prevents Diabetes-Induced Blood-Retinal Barrier Breakdown, Apoptosis, and Glial Reactivation in the Retina of db/db Mice
Cheung et al.
Diabetes 2005;54:3119-3125.
ABSTRACT | FULL TEXT  

The Ins2Akita Mouse as a Model of Early Retinal Complications in Diabetes
Barber et al.
IOVS 2005;46:2210-2218.
ABSTRACT | FULL TEXT  

Retinopathy in Monkeys with Spontaneous Type 2 Diabetes
Kim et al.
IOVS 2004;45:4543-4553.
ABSTRACT | FULL TEXT  

Death of Retinal Neurons in Streptozotocin-Induced Diabetic Mice
Martin et al.
IOVS 2004;45:3330-3336.
ABSTRACT | FULL TEXT  

Ultrastructural Aging of the RPE-Bruch's Membrane-Choriocapillaris Complex in the D-Galactose-Treated Mouse
Ida et al.
IOVS 2004;45:2348-2354.
ABSTRACT | FULL TEXT  

Effect of PKC{beta} on Retinal Oxygenation Response in Experimental Diabetes
Luan et al.
IOVS 2004;45:937-942.
ABSTRACT | FULL TEXT  

Regulation of the Early Subnormal Retinal Oxygenation Response in Experimental Diabetes by Inducible Nitric Oxide Synthase
Berkowitz et al.
Diabetes 2004;53:173-178.
ABSTRACT | FULL TEXT  

Abnormalities of Retinal Metabolism in Diabetes and Experimental Galactosemia: VII. Effect of Long-Term Administration of Antioxidants on the Development of Retinopathy
Kowluru et al.
Diabetes 2001;50:1938-1942.
ABSTRACT | FULL TEXT  

Pharmacological Inhibition of Diabetic Retinopathy: Aminoguanidine and Aspirin
Kern and Engerman
Diabetes 2001;50:1636-1642.
ABSTRACT | FULL TEXT  

Mouse models of angiogenesis, arterial stenosis, atherosclerosis and hemostasis
Carmeliet et al.
Cardiovasc Res 1998;39:8-33.
ABSTRACT | FULL TEXT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 1996 American Medical Association. All Rights Reserved.