A mouse model of diabetic retinopathy
T. S. Kern and R. L. Engerman
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
OBJECTIVE: To obtain a model of diabetic retinopathy to which modern
methods of genetic engineering may be applied, by determining the response
of 2 strains of mice to long-term galactose feeding. METHODS: Both C57BL/6
mice BALB/c mice were fed each of 2 galactose-rich diets (30% and 50%
galactose), and trypsin digests of their retinas were compared with those
of controls at durations of up to 26 months. RESULTS: The mortality rate in
galactose-fed animals was lower in C57BL/6 mice than in BALB/c mice, and
both strains tolerated the 30% galactose diet significantly better than the
50% galactose diet. In C57BL/6 mice fed 30% galactose for 21 to 26 months,
saccular microaneurysms were observed in the retina, together with
significant increases in the thickness of capillary basement membrane and
the prevalence of acellular capillaries and pericyte ghosts. The 50%
galactose diet caused significantly more acellular capillaries than normal
by 15 months, but excessive mortality precluded study at longer durations.
The frequency of acellular capillaries also was greater than normal in
BALB/c mice fed 30% galactose for 21 months. Retinal polyol levels in
galactose-fed mice were found to be lower than those in galactosemic rats.
CONCLUSION: The mouse may provide an inexpensive model suitable for in vivo
study of the pathogenesis of diabetic retinopathy using molecular
biological techniques.
Diabetic Retinopathy: Seeing Beyond Glucose-Induced Microvascular Disease
Antonetti et al.
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Berkowitz et al.
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Abnormalities of Retinal Metabolism in Diabetes and Experimental Galactosemia: VII. Effect of Long-Term Administration of Antioxidants on the Development of Retinopathy
Kowluru et al.
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Mouse models of angiogenesis, arterial stenosis, atherosclerosis and hemostasis
Carmeliet et al.
Cardiovasc Res 1998;39:8-33.
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