Identification of Glycosaminoglycans in Age-Related Macular Deposits

doi:10.1001/archopht.1996.01100140217021

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Vol. 114 No. 8, August 1996

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Identification of Glycosaminoglycans in Age-Related Macular Deposits

Mike Kliffen, MD; Cornelia M. Mooy, MD; Theo M. Luider, PhD; Jan G. M. Huijmans, PhD; Sonja Kerkvliet; Paulus T. V. M. de Jong, MD

Arch Ophthalmol. 1996;114(8):1009-1014.

Abstract

We investigated the presence and localization of glycosaminoglycansin basal laminar deposit and drusen in age-related maculopathy.Conventional histological staining techniques and monoclonalantibodies specific for several glycosaminoglycans were usedon paraffin-embedded human maculae. Furthermore, macular homogenateswere analyzed with two-dimensional electrophoresis. Quantitativeanalysis of glycosaminoglycans was done spectrophotometricallyusing dimethylmethylene blue. Immunohistochemically, all basallaminar deposit stained positive for chondroitin 4-sulfate andfocally positive for heparan sulfate proteoglycan. Drusen werenot stained with any of the monoclonal antibodies. With two-dimensionalelectrophoresis, it was demonstrated that macular extracts withand without age-related maculopathy contained chondroitin sulfate.Heparan sulfate was only expressed in maculae with age-relatedmaculopathy. The total amount of glycosaminoglycans was significantlyhigher in maculae with basal laminar deposit than in maculaewithout basal laminar deposit (P=.001). There were significantdifferences in the amount and composition of glycosaminoglycansbetween maculae with and without age-related maculopathy.

Author Affiliations

From the Departments of Ophthalmology (Drs Kliffen, Mooy, and de Jong), Pathology (Dr Mooy and Ms Kerkvliet), and Clinical Genetics (Drs Luider and Huijmans), Erasmus University, Rotterdam, the Netherlands.

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