You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


Advertisement

ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | RSS | Access Rights | Sign In


  Vol. 114 No. 6, June 1996 TABLE OF CONTENTS
  Online Only
 •  Online First Table of
Contents
  LABORATORY SCIENCES
 •Online Features
 This Article
 •References
 •Full text PDF
 • Reply to article
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Citing articles on Web of Science (6)
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Delicious Add to Digg Add to Facebook Add to Reddit Add to Technorati Add to Twitter What's this?

Hyalocytes Synthesize and Secrete Inhibitors of Retinal Pigment Epithelial Cell Proliferation In Vitro

Howard S. Lazarus, MD; Carl-Ludwig Schoenfeld, MD; Sharon Fekrat, MD; Steven Cohen; Alicia Carol; Gregory S. Hageman, PhD; Sean Hackett; Ying-Shan Chen, MD; Stanley A. Vinores, PhD; Peter A. Campochiaro, MD

Arch Ophthalmol. 1996;114(6):731-736.


Abstract



Background
Retinal pigment epithelial (RPE) cells that enter the vitreous in pathologic conditions, such as retinal detachment, may proliferate and contribute to the formation of epiretinal membranes.

Objective
To study whether hyalocytes, endogenous vitreous cells, play a role in modulating the proliferation of RPE cells.

Methods
Cell proliferation was measured by tritiated thymidine incorporation in density-arrested human RPE cells after incubation with media that had been conditioned by cultured bovine hyalocytes. Preliminary characterization of inhibitory activity in hyalocyteconditioned medium was performed, including blocking experiments with a neutralizing antibody to transforming growth factor-β2 (TGF-β) and proliferation assays that used MV-1-Lu mink lung epithelial cells. Northern blots were done to assess hyalocyte expression of TGF-β messenger RNA.

Results
Hyalocyte-conditioned medium inhibited tritiated thymidine incorporation in RPE cells and MV-1-Lu mink lung epithelial cells in the presence or absence of serum or protease inhibitors. A portion of the inhibitory activity was neutralized by an antibody directed against TGF-β. Northern blots of hyalocyte RNA demonstrated the presence of messenger RNA for TGF-β2. These data suggest that TGF-β is responsible for a portion of the inhibitory activity secreted by hyalocytes. Additional inhibitory activity is attributable to one or more low-molecular-weight molecules distinct from TGF-β.

Conclusion
Hyalocyte-conditioned medium inhibits RPE cell proliferation in vitro through TGF-β and at least one other molecule. Production of these factors by hyalocytes in vivo could provide a deterrent for epiretinal membrane formation that may be perturbed under pathologic conditions.



Author Affiliations



From The Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, Baltimore, Md (Drs Lazarus, Schoenfeld, Fekrat, Chen, Vinores, and Campochiaro, Messrs Cohen and Hackett, and Ms Carol); and Anheuser-Busch Eye Institute, Department of Ophthalmology, St Louis (Mo) University School of Medicine (Dr Hageman).



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Delicious Delicious   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Functional Properties of Hyalocytes under PDGF-Rich Conditions
Noda et al.
IOVS 2004;45:2107-2114.
ABSTRACT | FULL TEXT  

Retina-Specific Expression of PDGF-B Versus PDGF-A: Vascular Versus Nonvascular Proliferative Retinopathy
Mori et al.
IOVS 2002;43:2001-2006.
ABSTRACT | FULL TEXT  

TGF{beta}1-Dependent Contraction of Fibroblasts Is Mediated by the PDGF{alpha} Receptor
Ikuno and Kazlauskas
IOVS 2002;43:41-46.
ABSTRACT | FULL TEXT  

Laceration of the eye with a fishing hook
KROTT et al.
Br J Ophthalmol 1999;83:1194-1194.
FULL TEXT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | PHYSICIAN JOBS | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 1996 American Medical Association. All Rights Reserved.