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Immunohistochemical Analysis of Healing

Janet A Anderson, PhD; Perry S. Binder, MD; Michael E. Rock; Michael P. Vrabec, MD

Arch Ophthalmol. 1996;114(1):54-60.

Abstract
Objective
To analyze human corneal responses to excimer laser by immunohistochemistry.

Methods
Corneas cultured for 3 weeks after laser ablation or taken from patients 5 to 16 months after laser treatment were exposed to antibodies to β₄ integrin; types VII, IV, and III collagen; fibronectin; type I procollagen; and prolyl 4-hydroxylase. Antibody distributions were compared with those of normal corneas and unablated regions of treated corneas.

Results
After 3 weeks, distribution of β₄ integrin and types VII and IV collagen was patchy; heavy deposits of fibronectin appeared subepithelially. Keratocytes stained for prolyl 4-hydroxylase and type I procollagen. With increasing postoperative time, fibronectin diminished, and β₄ integrin and type VII collagen became linear; stromal staining of types III and IV collagen increased and then diminished.

Conclusions
Components of basement membrane, attachment complexes, and stromal matrix are synthesized shortly after laser treatment. Changes in these elements persist for 16 months in the human cornea.

Author Affiliations
From the National Vision Research Institute, San Diego, Calif (Drs Anderson and Binder and Mr Rock), and Department of Ophthalmology, The University of Vermont, Burlington (Dr Vrabec).; Dr Binder is a participant in a clinical research trial using the Visx Excimer Laser and directed by the Visx Co, Sunnyvale, Calif. Dr Vrabec is now affiliated with Valley Eye Associates, Appleton, Wis.

Footnotes
The authors have no commercial or proprietary interest in any products or companies discussed in this article.

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