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François Malecaze, MD, PhD; Simone Clamens; Valérie Simorre-Pinatel, MD; André Mathis, MD; Pascale Chollet, MD; Catherine Favard, MD; Francis Bayard, MD, PhD; Jean Plouet, MD, PhD

Arch Ophthalmol. 1994;112(11):1476-1482.

Abstract
Objective
To study the involvement of eight angiogenic growth factors that have been identified so far in the literature, especially vascular endothelial growth factor, in proliferative diabetic retinopathy.

Methods
Samples of neovascular membranes were obtained from diabetic patients; these samples, excised at vitrectomy, were used to study the expression of messenger RNA of the angiogenic factors by using the method of the reverse transcription-polymerase chain reaction. Vitreous aspirates that were taken from diabetic and control patients were used to quantify vascular endothelial growth factor-like activity with a competitive radioreceptor assay.

Results
Of the eight angiogenic factors studied, vascular endothelial growth factor was the only one that was always expressed in the samples of neovascular membranes. Furthermore, vascular endothelial growth factor receptor-binding activity was greater in vitreous aspirates that were obtained from diabetic patients than in samples that were taken from control patients (P<.01).

Conclusion
Vascular endothelial growth factor seems to be an appropriate candidate for mediating retinal diabetic neovascularization.

Author Affiliations
From the Laboratory of Molecular Biology, CNRS (Drs Malecaze, Simorre-Pinatel, Chollet, and Plouet), the Laboratory of Ophthalmology, (Drs Malecaze, Simorre-Pinatel, Mathis, and Chollet), University of Toulouse (France), the Laboratory of Endocrinology, National Institute of Health and Medical Research (INSERM) (Ms Clamens and Dr Bayard), Toulouse, France; and the Department of Ophthalmology, Hotel Dieu, Paris, France (Dr Favard).; Dr Malecaze is now with the Department of Ophthalmology, Hopital Purpan, Toulouse, France.

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