In Situ Characterization of the Human Hyalocyte

doi:10.1001/archopht.1994.01090220106031

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Vol. 112 No. 10, October 1994

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In Situ Characterization of the Human Hyalocyte

Howard S. Lazarus, MD; Gregory S. Hageman, PhD

Arch Ophthalmol. 1994;112(10):1356-1362.

Abstract

Objective
To provide more definitive evidence regarding the cellular originof hyalocytes and to establish a basis for their identificationin tissue specimens.

Methods
Hyalocytes were examined in situ in human eyes from 10 donors(aged 1 to 63 years) with use of a panel of antibodies and lectinsdirected against determinants associated with leukocytic andnonleukocytic cells.

Results
Hyalocytes express the leukocyte-associated antigens CD45, CD11a,and CD64, an antigen expressed constitutively only by monocytesand macrophages. However, they do not react with antibodiesagainst CD68, an antigen that is expressed by virtually alltissue macrophages, or CD 1 lb and CD 14, antigens that arevariably expressed by the monocyte/macrophage lineage. Theyalso react with antibodies against major histocompatibilitycomplex class II antigens and S100, both of which are expressedby a variety of cell types. The lectins Dolichos biflorus, Sambucusnigra, Griffonia simplicifolia, Limax flavus, and Ricinus communisI bind hyalocyte-associated glycoconjugates; no binding wasobserved with Arachis hypogaea or Glycine max.

Conclusions
These results provide strong evidence that hyalocytes belongto the monocyte/macrophage lineage but differ significantlyfrom other tissue macrophages in that they express S100 proteinand do not express CD68. This information should provide a basisfor the identification of hyalocytes in tissue specimens andan improved understanding of vitreoretinal pathologic features.

Author Affiliations

From The Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, Baltimore, Md (Dr Lazarus), and the Anheuser-Busch Eye Institute, Department of Ophthalmology, St Louis (Mo) University School of Medicine (Dr Hageman).

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