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Noburo Ando, MD; Harsha A. Sen, MD; Bruce A. Berkowitz, PhD; Charles A. Wilson, MD; Eugene de Juan, Jr, MD

Arch Ophthalmol. 1994;112(1):117-122.

Abstract
Objective
To determine the contribution of the break-down of the blood-retinal barrier (BRB) as measured with magnetic resonance imaging in the development of retinal detachment in an experimental model of proliferative vitreoretinopathy.

Methods
Contrast-enhanced magnetic resonance imaging was used to evaluate BRB breakdown in an intravitreal cell-injection model of proliferative vitreoretinopathy. Intravitreal injection of 2.5x10⁵ homologous dermal fibroblasts produced specific disruption of the inner, or vascular, BRB.

Results
Breakdown of the BRB was greatest in the first 3 days after injection, confirming previous work using fluorescein-based methods. Injection of 1 mg of intravitreal triamcinolone acetonide at the time of cell injection significantly reduced both BRB breakdown and the incidence of eventual traction retinal detachment. Eyes that did develop detachment had significantly greater leakage prior to its development than those that did not, regardless of steroid treatment.

Conclusions
Quantitation and definitive localization of BRB leakage with magnetic resonance imaging provides a better understanding of the relationship between BRB compromise and the development of retinal detachment in this frequently used model.

Author Affiliations
From Duke University Eye Center, Durham, NC (Drs Ando, Sen, Wilson, and de Juan) and the National Institute of Environmental Health Sciences, Research Triangle Park, NC (Dr Berkowitz). Drs Berkowitz and Wilson are currently with the Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas; Dr de Juan is now at The Wilmer Ophthalmological Institute, The Johns Hopkins Hospital, Baltimore, Md; Dr Ando has returned to the Department of Ophthalmology, Niigata (Japan) University; and Dr Sen is now at the Department of Ophthalmology, University of Kentucky, Lexington.

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