Prolonged localized tissue effects from 5-minute exposures to fluorouracil and mitomycin C

P. T. Khaw, J. W. Doyle, M. B. Sherwood, I. Grierson, G. Schultz and S. McGorray
Institute for Wound Research, J. Hillis Miller Health Center, University of Florida, Gainesville.

Rabbits undergoing full-thickness glaucoma filtering surgery were exposed for 5 minutes to one of three intraoperative treatments: (1) distilled water; (2) fluorouracil, 50 mg/mL; or (3) mitomycin C, 0.4 mg/mL. Tissue samples were taken from the subconjunctival and scleral tissues at the treated area and 90 degrees and 180 degrees from the center of the treated area and the adjacent cornea 2 mm from the limbus, 1 hour, 5 days, and 30 days postoperatively. The biopsy specimens were then placed in tissue culture media and the fibroblast outgrowths measured. Five-minute intraoperative treatments with fluorouracil resulted in a reversible delay of fibroblast outgrowths from treated subconjunctival and scleral tissues of just over 1 week in this model, whereas treatment with mitomycin C, 0.4 mg/mL, resulted in prolonged inhibition of at least 30 days. These effects were localized to the area treated. The many clinical implications of these findings are discussed.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

The Rho-Kinase Inhibitor H-1152P Suppresses the Wound-Healing Activities of Human Tenon's Capsule Fibroblasts In Vitro
Tura et al.
IOVS 2007;48:2152-2161.
ABSTRACT | FULL TEXT  

Single Perivascular Delivery of Mitomycin C Stimulates p21 Expression and Inhibits Neointima Formation in Rat Arteries
Granada et al.
Arterioscler. Thromb. Vasc. Bio. 2005;25:2343-2348.
ABSTRACT | FULL TEXT  

Prevention of Anterior Glottic Stenosis After Bilateral Vocal Fold Stripping With Mitomycin C
Roh and Yoon
Arch Otolaryngol Head Neck Surg 2005;131:690-695.
ABSTRACT | FULL TEXT  

Prolonged Antiscarring Effects of Ilomastat and MMC after Experimental Glaucoma Filtration Surgery
Wong et al.
IOVS 2005;46:2018-2022.
ABSTRACT | FULL TEXT  

Inhibitory Effect of Rapamycin on Corneal Neovascularization In Vitro and In Vivo
Kwon et al.
IOVS 2005;46:454-460.
ABSTRACT | FULL TEXT  

Histopathologic Features of Conjunctival Filtering Blebs
Francis et al.
Arch Ophthalmol 2005;123:166-170.
ABSTRACT | FULL TEXT  

Alkylphosphocholines: A New Therapeutic Option in Glaucoma Filtration Surgery
Eibl et al.
IOVS 2004;45:2619-2624.
ABSTRACT | FULL TEXT  

Problem of Dural Scarring in Recording From Awake, Behaving Monkeys: A Solution Using 5-Fluorouracil
Spinks et al.
J. Neurophysiol. 2003;90:1324-1332.
ABSTRACT | FULL TEXT  

Evaluation of Anti-TGF-{beta}2 Antibody as a New Postoperative Anti-scarring Agent in Glaucoma Surgery
Mead et al.
IOVS 2003;44:3394-3401.
ABSTRACT | FULL TEXT  

Matrix Metalloproteinase Inhibition Modulates Postoperative Scarring after Experimental Glaucoma Filtration Surgery
Wong et al.
IOVS 2003;44:1097-1103.
ABSTRACT | FULL TEXT  

Matrix Metalloproteinase Inhibition Modulates Fibroblast-Mediated Matrix Contraction and Collagen Production In Vitro
Daniels et al.
IOVS 2003;44:1104-1110.
ABSTRACT | FULL TEXT  

A new delivery system for 5-fluorouracil using prodrug and converting enzyme
Akimoto et al.
Br. J. Ophthalmol. 2002;86:581-586.
ABSTRACT | FULL TEXT  

Syndrome of Iron Pill Aspiration*
Lee et al.
Chest 2002;121:1355-1357.
ABSTRACT | FULL TEXT  

The Role of Mitomycin in the Prevention and Treatment of Scar Formation in the Pediatric Aerodigestive Tract: Friend or Foe?
Rahbar et al.
Arch Otolaryngol Head Neck Surg 2002;128:401-406.
ABSTRACT | FULL TEXT  

Apoptosis Gene Expression and Death Receptor Signaling in Mitomycin-C-Treated Human Tenon Capsule Fibroblasts
Crowston et al.
IOVS 2002;43:692-699.
ABSTRACT | FULL TEXT  

Topical Mitomycin Application After Laryngotracheal Reconstruction: A Randomized, Double-blind, Placebo-Controlled Trial
Hartnick et al.
Arch Otolaryngol Head Neck Surg 2001;127:1260-1264.
ABSTRACT | FULL TEXT  

Glaucoma Filtration Surgery using Amniotic Membrane Transplantation
Barton et al.
IOVS 2001;42:1762-1768.
ABSTRACT | FULL TEXT  

Early Breaking Strength of Repaired Flexor Tendon Treated with 5-Fluorouracil
CEROVAC et al.
J Hand Surg Eur Vol 2001;26:220-223.
ABSTRACT  

Sponge delivery variables and tissue levels of 5-fluorouracil
Wilkins et al.
Br. J. Ophthalmol. 2000;84:92-97.
ABSTRACT | FULL TEXT  

Human Anti-Transforming Growth Factor-{beta}2 Antibody: A New Glaucoma Anti-Scarring Agent
Cordeiro et al.
IOVS 1999;40:2225-2234.
ABSTRACT | FULL TEXT  

Effects of single, short term exposures of human retinal pigment epithelial cells to thiotepa or 5-fluorouracil: implications for the treatment of proliferative vitreoretinopathy
Kon et al.
Br. J. Ophthalmol. 1998;82:554-560.
ABSTRACT | FULL TEXT  

Late-Onset Bleb Leaks After Glaucoma Filtering Surgery
Greenfield et al.
Arch Ophthalmol 1998;116:443-447.
ABSTRACT | FULL TEXT  

Correction of lower eyelid retraction in thyroid eye disease: a randomised controlled trial of retractor tenotomy with adjuvant antimetabolite versus scleral graft
Olver et al.
Br. J. Ophthalmol. 1998;82:174-180.
ABSTRACT | FULL TEXT  

Aging and the cornea
FARAGHER et al.
Br. J. Ophthalmol. 1997;81:814-817.
FULL TEXT  

Trabeculectomy with intraoperative sponge 5-fluorouracil in Afro-Caribbeans
Lachkar et al.
Br. J. Ophthalmol. 1997;81:555-558.
ABSTRACT | FULL TEXT