Immunoreactivity of Exfoliation Material for the Cell Adhesion—Related HNK-1 Carbohydrate Epitope
Marita Uusitalo, MD;
Tero Kiveldä, MD;
Ahti Tarkkanen, MD
Arch Ophthalmol. 1993;111(10):1419-1423.
Abstract
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Objective
To study the presence of the HNK-1 epitope in exfoliation material.
Methods
Twenty-six formalin-fixed, paraffinembedded human eyes with exfoliation syndrome and 30 control eyes were studied immunohistochemically with monoclonal antibodies HNK-1 and VC1.1 to the HNK-1 epitope.
Results
Exfoliation material reacted consistently with antibodies to the HNK-1 epitope. The zonular lamella of the lens, inner surface of the nonpigmented ciliary epithelium, and inner connective tissue layer of the ciliary body were also labeled, but the lens capsule, epithelium, and zonules were not immunoreactive. Several blood vessels of the iris showed granular immunoreaction beneath the endothelium in all exfoliation eyes and in 11 (37%) of 30 control eyes, representing older age groups.
Conclusions
The zonular lamella, nonpigmented ciliary epithelium, or the inner connective tissue layer may be responsible for the HNK-1 epitope in exfoliation material. Since this epitope is shared by many cell-adhesion molecules, its presence in exfoliation material might be of pathogenetic significance to the formation of the deposits.
Author Affiliations
From the Department of Ophthalmology, Helsinki (Finland) University Central Hospital. The authors have no financial interest in the products or methods described in this study.
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