Altered Epithelial-Basement Membrane Interactions in Diabetic Corneas

doi:10.1001/archopht.1992.01080160115045

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Vol. 110 No. 4, April 1992

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Altered Epithelial-Basement Membrane Interactions in Diabetic Corneas

Dimitri T. Azar, MD; Sandra J. Spurr-Michaud, MS; Ann S. Tisdale, MS; Ilene K. Gipson, PhD

Arch Ophthalmol. 1992;110(4):537-540.

Abstract

• Morphologic alterations of the extracellular matrix indiabetes include thickening of the basement membrane and, asevidenced in the corneal epithelium, a decrease in the areaof the basal cell membrane occupied by hemidesmosomes. To determineif the abnormal basement membrane in diabetes has altered epithelialrecognition of sites for hemidesmosome formation and if diabeticepithelium can recognize sites for hemidesmosome formation onnormal basement membrane, we recombined epithelial sheets andcorneal stromas with denuded basement membranes in vitro usingdiabetic and control corneas. In recombinations of diabeticepithelium on normal stromas, the area of the basal cell membraneoccupied by hemidesmosomes was 10.7%, significantly less thancontrol recombinations (19.5%). Similarly, in recombinationsof normal epithelium on diabetic stromas, the value (8.6%) wassignificantly decreased from control. These data may indicatealtered cell-extracellular matrix interactions in diabetes.

Author Affiliations

From the Eye Research Institute (Drs Azar and Gipson, and Mss Spurr-Michaud and Tisdale), the Massachusetts Eye and Ear Infirmary (Dr Azar), and the Department of Ophthalmology (Drs Azar and Gipson), Harvard Medical School, Boston, Mass.

Footnotes

Accepted for publication November 8, 1991.

Reprint requests to Eye Research Institute, 20 Staniford St,Boston, MA 02114 (Dr Gipson).

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