 |
|
Immunocytochemical Labeling of Cells in Cortical Vitreous From Patients With Premacular Hole Lesions
Peter A. Campochiaro, MD;
Ellen Van Niel;
Stanley A. Vinores, PhD
Arch Ophthalmol. 1992;110(3):371-377.
Abstract
| |
• We performed electron immunocytochemical staining for cytokeratins and glial fibrillary acidic protein on cortical vitreous obtained at the time of vitrectomy from two patients with premacular hole lesions. The specimens were thought to represent cortical vitreous because each consisted of a sheet of tissue that, in addition to being firmly attached around the foveal lesion, was attached around the disc and extended well into the periphery. The specimens contained a moderate number of cells and an abundant extracellular matrix. Most of the cells were found singly or in small clusters on the surface of the matrix. Preembedding immunostaining on one specimen showed several cells that stained for cytokeratins and several that stained for glial fibrillary acidic protein. The majority of the cells on the matrix appeared to express one of these two intermediatefilament proteins. Postembedding immunogold double labeling on both specimens showed that most cells were labeled for either glial fibrillary acidic protein or cytokeratins, but there were a few cells that unequivocally expressed both proteins simultaneously. These data suggest that the cortical vitreous of patients with some premacular hole lesions contains retinal pigment epithelial and glial cells that may contribute to abnormal vitreoretinal adherence and could play a role in macular hole formation.
Author Affiliations
From the Department of Ophthalmology, University of Virginia Medical Center, Charlottesville. Drs Campochiaro and Vinores are now with The Wilmer Institute, The Johns Hopkins Hospital, Baltimore, Md.
Footnotes
Accepted for publication September 11, 1991.
Presented at the Association for Research in Vision and Ophthalmology Meeting, Sarasota, Fla, April 28, 1991.
Reprint requests to The Wilmer Institute, The Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD 21205 (Dr Campochiaro).
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Cellular Migration Associated With Macular Hole: A New Method for Comprehensive Bird's-Eye Analysis of the Internal Limiting Membrane
Hisatomi et al.
Arch Ophthalmol 2006;124:1005-1011.
ABSTRACT
| FULL TEXT
Immunocytochemical Characterization of Macular Hole Opercula
Ezra et al.
Arch Ophthalmol 2001;119:223-231.
ABSTRACT
| FULL TEXT
Idiopathic full thickness macular hole: natural history and pathogenesis
EZRA
Br J Ophthalmol 2001;85:102-109.
FULL TEXT
Macular Hole Opercula: Ultrastructural Features and Clinicopathological Correlation
Ezra et al.
Arch Ophthalmol 1997;115:1381-1387.
ABSTRACT
Tissue Analysis From Two Patients With Premacular Hole Lesions
Sebag
Arch Ophthalmol 1993;111:22-22.
ABSTRACT
|
