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Dimitri T. Azar, MD; Sandra J. Spurr-Michaud, MS; Ann S. Tisdale, MS; Mary Beth Moore, MD; Ilene K. Gipson, PhD

Arch Ophthalmol. 1991;109(9):1279-1284.

Abstract
• Ten epikeratoplasty lenticules removed after surgery were obtained for immunohistochemical and electron microscopic analysis to determine the pattern of re-formation of corneal epithelial adhesion structures. Antibodies to laminin and type VII collagen were used to determine the presence of basement membrane and anchoring fibrils, respectively. Electron micrographs were used to determine the percentage of basal cell membrane occupied by hemidesmosomes, the area of basal lamina per 100 µg of basal cell membrane, and the average maximum depth of penetration of anchoring fibrils into the stroma. Nine normal corneas served as controls. Compared with normal corneas (24.5% of basal cell membrane occupied by hemidesmosomes; 32.0 µm² basal lamina per 100 µm of basal cell membrane), lenticules removed for optical reasons had near-normal hemidemosomes as early as 10 weeks following surgery (mean, 20.3%). The area of basement membrane was reduced (16 µm² basal lamina per 100 µm of basement cell membrane). During the course of 2 to 3 years, irregularities and duplications of the basement membrane were noted. Compared with normal corneas, the two lenticules removed for persistent defects had a marked reduction of hemidesmosomes and basement membrane present under epithelium at 3 and 4 weeks (9.6% of basal cell membrane occupied by hemidesmosomes and 13.6 µm² basal lamina per 100 µm of basal cell membrane, and 5.4% of basal cell membrane occupied by hemidesmosomes and 7.2 µm² basal lamina per 100 µm of basal cell membrane, respectively).

Author Affiliations
From the Eye Research Institute (Drs Azar and Gipson and Mss Spurr-Michaud and Tisdale) and the Massachusetts Eye and Ear Infirmary (Dr Azar), Boston, Mass; the Department of Ophthalmology, University of Texas at Dallas (Dr Moore); and the Department of Ophthalmology, Harvard Medical School, Boston (Drs Azar and Gipson).

Footnotes
Accepted for publication May 30, 1991.

Reprint requests to the Eye Research Institute, 20 Staniford St, Boston, MA 02114 (Dr Gipson).

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