A simple method of determining the time course of timolol's effects on aqueous flow in humans
M. Mori and M. Araie
Department of Ophthalmology, University of Tokyo, School of Medicine, Japan.
We have devised a simple method of determining the time course of the
effects of timolol maleate on aqueous flow using changes in aqueous protein
concentrations. Aqueous protein concentrations were measured with a laser
flare-cell meter. First, the value of the coefficient of protein entry into
the anterior chamber (kin) was determined in both eyes of 10 normal
subjects. One week later, aqueous protein concentration changes were
measured in both eyes of the same subjects before and after instillation of
timolol in one eye. From these changes and the value of kin, changes in the
aqueous flow rate were calculated using an equation formulating protein
kinetics in the anterior chamber. Results of a separate experiment showed
that instillation of topical timolol did not change the kin, justifying use
of the kin as determined without considering timolol in the calculation.
The calculated flow rate had a maximum decrease of 30% +/- 10% (mean +/-
SEM, n = 10) 4 hours after instillation in treated eyes and of 10% +/- 6% 3
hours after instillation in fellow, untreated eyes.
