Intraocular irrigating solutions and permeability of the blood-aqueous barrier
M. Araie, E. Shirasawa and T. Ohashi
Department of Ophthalmology, University of Tokyo, School of Medicine, Japan.
The effects of intraocular irrigating solutions on the blood-aqueous
barrier were studied using rabbits injected with fluorescein
isothiocyanate-labeled rabbit albumin. Two days after the injection, the
anterior chamber was perfused for 121 minutes with one of the following
solutions: normal saline, a commercially available citrate-acetate
bicarbonate solution, or a glucose glutathione bicarbonate solution; the
amount of fluorescein isothiocyanate-labeled rabbit albumin collected in
the outflowing perfusate standardized to its plasma concentration was
adopted as a blood-aqueous barrier permeability index. Of the three
solutions, normal saline showed the highest permeability index and glucose
glutathione bicarbonate solution showed the lowest. The effects of oxidized
glutathione on the blood-aqueous barrier were also studied, using solutions
containing various concentrations of oxidized glutathione. The permeability
index for the solution containing 0.3 mmol/L of oxidized glutathione was
significantly smaller than that for the solution without oxidized
glutathione, suggesting that oxidized glutathione has a beneficial effect
on the integrity of the blood-aqueous barrier.
